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Mol Cell Endocrinol. 2014 Mar 5;383(1-2):103-10. doi: 10.1016/j.mce.2013.12.001. Epub 2013 Dec 8.

YY1 represses the transcriptional activity of Runx2 in C2C12 cells.

Author information

1
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, South Korea.
2
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, South Korea. Electronic address: kwanglee@chonnam.ac.kr.

Abstract

Runx2 is a major transcription factor that induces osteoblast differentiation by bone morphogenetic proteins (BMPs). Conversely, YY1 is a transcription factor that inhibits BMP2-induced cell differentiation. Until now, there has been no understanding of how osteoblast differentiation by Runx2 and YY1 is regulated. In this study we focused on the relationship between Runx2 and YY1. We confirmed that alkaline phosphatase staining is repressed by YY1. Runx2 interacted with YY1 through Runt and the C-terminus domain of Runx2. YY1 markedly repressed the Runx2-mediated enhancement of transcriptional activity on the osteocalcin and alkaline phosphatase promoters. Knockdown of YY1 enhanced BMP2- and Runx2-induced osteoblast differentiation. YY1 decreased Runx2 DNA binding affinity. The results indicate that YY1 represses osteoblast differentiation by an interaction with Runx2 and inhibits the transcriptional activity of Runx2.

KEYWORDS:

DNA binding affinity; Runx2; Transcriptional activity; YY1

PMID:
24325869
DOI:
10.1016/j.mce.2013.12.001
[Indexed for MEDLINE]
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