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Diagn Microbiol Infect Dis. 2014 Feb;78(2):129-31. doi: 10.1016/j.diagmicrobio.2013.08.013. Epub 2013 Dec 8.

Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria.

Author information

1
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. Electronic address: jbranda@partners.org.
2
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
3
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
4
Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA.
5
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
6
Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, Lake Success, NY, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.
7
Barnes Jewish Hospital, St. Louis, MO, USA.
8
Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.
9
Barnes Jewish Hospital, St. Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA.

Abstract

The VITEK MS v2.0 MALDI-TOF mass spectrometry system's performance in identifying fastidious gram-negative bacteria was evaluated in a multicenter study. Compared with the reference method (DNA sequencing), the VITEK MS system provided an accurate, species-level identification for 96% of 226 isolates; an additional 1% were accurately identified to the genus level.

KEYWORDS:

Fastidious; Gram-negative bacilli; Identification; MALDI-TOF MS; VITEK

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