Format

Send to

Choose Destination
J Proteome Res. 2014 Jan 3;13(1):321-3. doi: 10.1021/pr4011052. Epub 2013 Dec 16.

Accounting for population variation in targeted proteomics.

Author information

1
Biological Sciences Division, Pacific Northwest National Laboratory , 902 Battelle Boulevard, Richland, Washington 99532, United States.

Abstract

Individual proteomes typically differ from the reference human proteome at ∼10,000 single amino acid variants. When viewed on the population scale, this individual variation results in a wide variety of protein sequences. In targeted proteomics experiments, such variability can confound accurate protein quantification. To assist researchers in identifying target peptides with high variability within the human population, we have created the Population Variation plug-in for Skyline, which provides easy access to the polymorphisms stored in dbSNP. Given a set of peptides, the tool reports minor allele frequency for common polymorphisms. We highlight the importance of considering genetic variation by applying the tool to public data sets.

PMID:
24320210
PMCID:
PMC3923399
DOI:
10.1021/pr4011052
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center