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Nat Med. 2014 Jan;20(1):93-7. doi: 10.1038/nm.3416. Epub 2013 Dec 8.

Magnetic resonance imaging of tumor glycolysis using hyperpolarized 13C-labeled glucose.

Author information

1
Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
2
Department of Biochemistry, University of Cambridge, Cambridge, UK.
3
1] Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK. [2] Department of Biochemistry, University of Cambridge, Cambridge, UK. [3].

Abstract

In this study, we monitored glycolysis in mouse lymphoma and lung tumors by measuring the conversion of hyperpolarized [U-2H, U-13C]glucose to lactate using 13C magnetic resonance spectroscopy and spectroscopic imaging. We observed labeled lactate only in tumors and not in surrounding normal tissue or other tissues in the body and found that it was markedly decreased at 24 h after treatment with a chemotherapeutic drug. We also detected an increase in a resonance assigned to 6-phosphogluconate in the pentose phosphate pathway. This technique could provide a new way of detecting early evidence of tumor treatment response in the clinic and of monitoring tumor pentose phosphate pathway activity.

PMID:
24317119
PMCID:
PMC3886895
DOI:
10.1038/nm.3416
[Indexed for MEDLINE]
Free PMC Article

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