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J Reprod Dev. 2014 Mar 7;60(1):78-82. Epub 2013 Dec 7.

Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines.

Author information

1
Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, Japan.

Abstract

This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with or without LIF contributed to the higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission competency of the chimeric rats was not influenced by the profile of ES cell lines until their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat strain used as the blastocyst donor, such as the WI strain, was a possible factor negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines were established, all lines were found to be germline-competent by a progeny test in chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a beneficial role in the establishment of "genuine" rat ES cell lines.

PMID:
24317016
PMCID:
PMC3958585
[Indexed for MEDLINE]
Free PMC Article

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