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J Pharm Biomed Anal. 2014 Feb;89:257-67. doi: 10.1016/j.jpba.2013.11.016. Epub 2013 Nov 19.

Metabolite profiling of polyphenols in Vaccinium berries and determination of their chemopreventive properties.

Author information

1
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 183, 41125 Modena, Italy.
2
Department of Food Science-LS9 Interlab Group, University of Parma, Via G.B. Usberti 95/a, 43134 Parma, Italy.
3
Department of Life Sciences and Biotechnologies-LT Terra&Acqua Tech UR7, University of Ferrara, Corso Ercole I d'Este 32, 44121 Ferrara, Italy.
4
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 183, 41125 Modena, Italy. Electronic address: federica.pellati@unimore.it.

Abstract

A detailed investigation on the chemical composition and chemopreventive activity of Vaccinium floribundum Kunth berries was carried out in comparison with Vaccinium myrtillus L. Berry polyphenols were extracted by using two sequential dynamic maceration steps, which enabled to maximize the yields of secondary metabolites. In particular, phenolic acids and flavonols were extracted from berries using ethyl acetate (EtOAc), whereas anthocyanins were extracted from the residue with 0.6M HCl in methanol (MeOH). The analysis of secondary metabolites in berry extracts was performed by means of two specific HPLC methods. Phenolic acids and flavonols were analyzed on an Ascentis C18 column (250mm×4.6mm I.D., 5μm), with a gradient mobile phase composed of 0.1M HCOOH in H2O and ACN. Anthocyanin analysis was carried out on a Zorbax SB-C18 column (150mm×4.6mm I.D., 5μm), with a gradient mobile phase composed of H2O-HCOOH (9:1, v/v) and MeOH-H2O-HCOOH (5:4:1, v/v/v). Detection was performed by UV/DAD, MS and MS(2). The polyphenol composition of V. floribundum and V. myrtillus was studied in detail. The samples of V. floribundum analyzed in this study had a much higher content of both phenolic acids and flavonols in comparison with V. myrtillus (mean value 41.6±10.2 and 13.7±0.2mg/100g FW, respectively), while V. myrtillus showed a higher amount of anthocyanins if compared with V. floribundum (568.8±8.8 and mean value 376.2±49.9mg/100gFW, respectively). The extracts gave negative results in antimutagenic assays against carcinogens 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) and 4-nitroquinoline-1-oxide (4-NQO), while they performed similarly in both ABTS(+) and DPPH antioxidant assays.

KEYWORDS:

2,2-diphenyl-1-picrylhydrazyl; 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); 2-amino-3,4-dimethylimidazo[4,5-f]quinoline; 4-NQO; 4-nitroquinoline-1-oxide; ABTS(+); Antioxidant; Berries; DPPH; HPLC; MeIQ; Polyphenols; Vaccinium

PMID:
24316426
DOI:
10.1016/j.jpba.2013.11.016
[Indexed for MEDLINE]
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