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Free Radic Biol Med. 2014 Mar;68:205-19. doi: 10.1016/j.freeradbiomed.2013.11.023. Epub 2013 Dec 4.

Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin.

Author information

1
Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain.
2
Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université, Case 906, 13288 Marseille cedex 9, France.
3
Grupo de Bioquímica y Señalización Celular, Departamento de Biología Experimental, Universidad de Jaén, 23071 Jaén, Spain.
4
Center for Biomedical Research of La Rioja, 26006 Logroño, Spain.
5
Unidad Funcional de Investigación en Enfermedades Crónicas, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
6
Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
7
Center for Biosciences at Novum, Department of Biosciences and Nutrition, Karolinska Institute, S-14183 Huddinge, Sweden.
8
Centro Andaluz de Biología del Desarrollo, Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, 41013 Sevilla, Spain; Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, 41013 Sevilla, Spain. Electronic address: amiranda-ibis@us.es.

Abstract

Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections.

KEYWORDS:

Caenorhabditis elegans; Candida albicans; Intestine; Pathogen infection; Photorhabdus luminescens; Stress; Thioredoxin

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