Format

Send to

Choose Destination
Gene. 2014 Feb 10;535(2):140-9. doi: 10.1016/j.gene.2013.11.047. Epub 2013 Dec 3.

Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera.

Author information

1
Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China; Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing,100081, PR China.
2
Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
3
Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China. Electronic address: shshen@ibcas.ac.cn.

Abstract

The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR. Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the DRE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species.

KEYWORDS:

ABA; ABA-responsive element; ABRE; AP2/EREBP-domain; AP2/ERF; APETALA 2/ERE binding factor; Broussonetia papyrifera; C-repeat binding factor; CBF; CaMV; DRE; DRE-binding protein; DRE/CRT; DREB; GFP; RACE; Salt and cold tolerance; Woody plant; abscisic acid; cauliflower mosaic virus; dehydration-responsive element; dehydration-responsive element/C-repeat; green fluorescent protein; mDRE; mutant dehydration-responsive element; rapid amplification of cDNA ends

PMID:
24315817
DOI:
10.1016/j.gene.2013.11.047
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center