Format

Send to

Choose Destination
BMC Res Notes. 2013 Dec 5;6:513. doi: 10.1186/1756-0500-6-513.

Online exercise for the design and simulation of PCR and PCR-RFLP experiments.

Author information

1
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria-Gasteiz, Spain. Joseba.bikandi@ehu.es.

Abstract

BACKGROUND:

Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism of PCR products (PCR-RFLP) are extensively used molecular biology techniques. An exercise for the design and simulation of PCR and PCR-RFLP experiments will be a useful educational tool.

FINDINGS:

An online PCR and PCR-RFLP exercise has been create that requires users to find the target genes, compare them, design primers, search for restriction endonucleases, and finally to simulate the experiment. Each user of the service is randomly assigned a gene from Escherichia coli; to complete the exercise, users must design an experiment capable of distinguishing among E. coli strains. By applying the experimental procedure to all completely sequenced E. coli, a basic understanding of strain comparison and clustering can also be acquired. Comparison of results obtained in different experiments is also very instructive.

CONCLUSIONS:

The exercise is freely available at http://insilico.ehu.es/edu.

PMID:
24314313
PMCID:
PMC4029544
DOI:
10.1186/1756-0500-6-513
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center