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Nat Photonics. 2013 Feb 1;7(2):93-101.

Advances in multiphoton microscopy technology.

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1
Center for Microintegrated Optics for Advanced Bioimaging and Control, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA. ; Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA.

Abstract

Multiphoton microscopy has enabled unprecedented dynamic exploration in living organisms. A significant challenge in biological research is the dynamic imaging of features deep within living organisms, which permits the real-time analysis of cellular structure and function. To make progress in our understanding of biological machinery, optical microscopes must be capable of rapid, targeted access deep within samples at high resolution. In this Review, we discuss the basic architecture of a multiphoton microscope capable of such analysis and summarize the state-of-the-art technologies for the quantitative imaging of biological phenomena.

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