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Cold Spring Harb Protoc. 2013 Dec 1;2013(12):1186-9. doi: 10.1101/pdb.prot079426.

Preparing size markers for gel electrophoresis.

Abstract

Here we present two simple methods for preparing radiolabeled size markers for gel electrophoresis. The first procedure describes the generation of an RNA marker ladder by the alkaline hydrolysis of (32)P 5'- or 3'-end-labeled RNA. The second procedure describes the labeling of DNA fragments produced by digestion of pBR322 with the restriction enzyme MspI.

PMID:
24298031
DOI:
10.1101/pdb.prot079426
[Indexed for MEDLINE]
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