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Appl Biochem Biotechnol. 2014 Feb;172(4):1938-52. doi: 10.1007/s12010-013-0653-9. Epub 2013 Dec 3.

L-lactate production from seaweed hydrolysate of Laminaria japonica using metabolically engineered Escherichia coli.

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Department of Chemical and Biological Engineering, Korea University, Seoul, 136-713, Republic of Korea.


Renewable and carbon neutral, marine algal biomass could be an attractive alternative substrate for the production of biofuel and various biorefinery products. Thus, the feasibility of brown seaweed (Laminaria japonica) hydrolysate as a carbon source was investigated here for L-lactate production. This work reports the homofermentative route for L-lactate production by introducing Streptococcus bovis/equinus L-lactate dehydrogenase in an engineered Escherichia coli strain where synthesis of the competing by-product was blocked. The engineered strain utilized both glucose and mannitol present in the hydrolysate under microaerobic condition and produced 37.7 g/L of high optical purity L-lactate at 80 % of the maximum theoretical value. The result shown in this study implies that algal biomass would be as competitive with lignocellulosic biomass in terms of lactic acid production and that brown seaweed can be used as a feedstock for the industrial production of other chemicals.

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