Format

Send to

Choose Destination
Dev Comp Immunol. 2014 May;44(1):50-8. doi: 10.1016/j.dci.2013.11.013. Epub 2013 Dec 1.

Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes.

Author information

1
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello, 6, 36208 Vigo, Spain.
2
Interdisciplinary Center of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal; Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
3
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello, 6, 36208 Vigo, Spain. Electronic address: cgestal@iim.csic.es.

Abstract

The hemocytes of Octopus vulgaris were morphologically and functionally characterized. Light and electron microscopy (TEM and SEM), and flow cytometry analyses revealed the existence of two hemocyte populations. Large granulocytes showed U-shaped nucleus, a mean of 11.6 μm±1.2 in diameter with basophilic granules, polysaccharide and lysosomic deposits in the cytoplasm. Small granulocytes measured a mean of 8.1 μm±0.7 in diameter, and have a round nucleus occupying almost the entire cell and few or not granules in the cytoplasm. Flow cytometry analysis showed that large granulocytes are the principal cells that develop phagocytosis of latex beads (rising up to 56%) and ROS after zymosan stimulation. Zymosan induced the highest production of both ROS and NO. This study is the first tread towards understanding the O. vulgaris immune system by applying new tools to provide a most comprehensive morpho-functional study of their hemocytes.

KEYWORDS:

Flow cytometry; Functional characterization; Hemocytes; Immune defense; Octopus vulgaris

PMID:
24296436
DOI:
10.1016/j.dci.2013.11.013
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center