Format

Send to

Choose Destination
Neuroscience. 2014 Feb 14;259:84-93. doi: 10.1016/j.neuroscience.2013.11.047. Epub 2013 Dec 1.

Neonatal olfactory bulbectomy enhances locomotor activity, exploratory behavior and binding of NMDA receptors in pre-pubertal rats.

Author information

1
Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico; Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montréal, QC H4H 1R3, Canada. Electronic address: gonzaloflores56@gmail.com.
2
Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, San Manuel, 72570 Puebla, Mexico.
3
Laboratorio de Neurociencia Celular y Plasticidad, Universidad Pablo de Olavide, Sevilla, Spain.
4
Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montréal, QC H4H 1R3, Canada.

Abstract

In this study, we investigated the effect of neonatal olfactory bulbectomy (nOBX) on behavioral paradigms related to olfaction such as exploratory behavior, locomotor activity in a novel environment and social interaction. We also studied the effect of nOBX on the activity of the N-methyl-d-aspartate (NMDA) subtype of glutamate receptors during development. The behavioral effects of nOBX (postnatal day 7, PD7) were investigated in pre- (PD30) and post-pubertal (PD60) Wistar rats. NMDA receptor activity was measured with [(125)I]MK-801 in the brain regions associated with the olfactory circuitry. A significant increase in the novelty-induced locomotion was seen in the pre-pubertal nOBX rats. Although the locomotor effect was less marked than in pre-pubertal rats, the nOBX rats tested post-pubertally failed to habituate to the novel situation as quickly as the sham- and normal- controls. Pre-pubertally, the head-dipping behavior was enhanced in nOBX rats compared with sham-operated and normal controls, while normal exploratory behavior was observed between groups in adulthood. In contrast, social interaction was increased in post-pubertal animals that underwent nOBX. Both pre- and post-pubertal nOBX rats recovered olfaction. Interestingly, pre-pubertal rats showed a significant increase in the [(125)I]MK-801 binding in the piriform cortex, dorsal hippocampus, inner and outer layers of the frontal cortex and outer layer of the cingulate cortex. At post-pubertal age, no significant differences in [(125)I]MK-801 binding were observed between groups at any of the brain regions analyzed. These results suggest that nOBX produces pre-pubertal behavioral disturbances and NMDA receptor changes that are transitory with recovery of olfaction early in adulthood.

KEYWORDS:

ANOVA; BL; Cg; DG; EDTA; HBT; MK-801; N-methyl-d-aspartate; NAcc; NMDA; NMDA receptor; OBX; OF; OT; PBS; PD; PFC; RMS; SI; SVZ; ablation of the olfactory bulbs; analysis of variance; basolateral; cingulate cortex; dPFC; dentate gyrus; dizocilpine; dorsal part of the prefrontal cortex; ethylenediaminetetraacetic acid; hole board test; limbic system; locomotion; nOBX; neonatal ablation of the olfactory bulbs; neonatal bilateral ablation of the olfactory bulbs; nucleus accumbens; olfactory bulbectomy and depression; olfactory tubercle; open field; phosphate saline solution; postnatal day; prefrontal cortex; rostral migratory stream; social interaction; subventricular zone

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center