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Methods Cell Biol. 2013;118:235-42. doi: 10.1016/B978-0-12-417164-0.00014-8.

Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy.

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Live Cell Molecular Imaging Research Team, Extreme Photonics Research Group, RIKEN Center for Advanced Photonics, Wako, Saitama, Japan.


Super-resolution confocal live imaging microscopy we developed provides cutting-edge high-speed live cell imaging at high space resolution. With this technology we are now able to observe details of membrane traffic events, including behaviors of small vesicles, cisternal maturation of the Golgi apparatus, and membrane segregation within a compartment.


Cisternal maturation; Live imaging; Multicolor visualization; Spinning disk confocal; Super-resolution

[Indexed for MEDLINE]

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