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In Vivo. 2013 Nov-Dec;27(6):747-53.

Photodynamic diagnosis of colitis-associated dysplasia in a mouse model after oral administration of 5-aminolevulinic acid.

Author information

1
Department of Endoscopy, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan. tkato@jikei.ac.jp.

Abstract

BACKGROUND:

Detection of dysplastic lesions in mouse colonic tissue was investigated by accumulation of photosensitizer protoporphyrin IX (PpIX) induced by oral administration of 5-aminolevulinic acid (5-ALA), a precursor of PplX.

MATERIALS AND METHODS:

Inflammatory oncogenesis was induced in C57BL/6J-Apc(Min) (Apc(Min/+)) mouse by oral administration of dextran sodium sulfate (DSS). After oral administration of 5-ALA, the colonic tissue was observed by autofluorescent stereoscopy and histopathological examination. The localization of fluorescence signals in the colonic tissue was determined with a mobile ultraviolet Xe lamp light.

RESULTS:

Several small polypoid lesions were found in the mucosal layer of DSS-treated Apc(Min/+) mice. Strong reddish ring-shaped fluorescence signals of PpIX, at 635 nm measured by a spectrum analyzer, were observed on the mucosal surface of all protruding lesions that were confirmed to be histopathologically dysplastic.

CONCLUSION:

Photodynamic diagnosis with 5-ALA was useful in detecting dysplastic lesions in the colonic mucosa in a mouse model.

KEYWORDS:

5-aminolevulinic acid; Photodynamic diagnosis; colitis-associated dysplasia; ulcerative colitis

PMID:
24292578
[Indexed for MEDLINE]
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