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Acta Biomater. 2014 Mar;10(3):1360-9. doi: 10.1016/j.actbio.2013.11.017. Epub 2013 Nov 27.

Platelet-derived growth-factor-releasing aligned collagen-nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells.

Author information

1
Microencapsulation and Nanomaterials Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA. Electronic address: xcheng@swri.org.
2
Microencapsulation and Nanomaterials Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA.
3
Department of Orthopedics, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229, USA.
4
Materials Engineering Department, Southwest Research Institute, 6220 Culebra Rd, San Antonio, TX 78238, USA.
5
Extremity Trauma and Regenerative Medicine Program, US Army Institute of Surgical Research, 3698 Chambers Pass, Fort Sam Houston, TX 78234, USA.

Abstract

In order to enhance the healing potential of an injured tendon, we have prepared a novel biomimetic aligned collagen-nanoparticle (NP) composite fiber using an electrochemical process. The aligned collagen-NP composite fiber is designed to affect the cellular activity of adipose-derived stem cells (ADSCs) through two different ways: (i) topographic cues from the alignment of collagen fibril and (ii) controlled release of platelet-derived growth factors (PDGFs) from the NPs. PDGF released from collagen-NP fibers significantly enhanced the proliferation of ADSCs when tested for up to 7 days. Moreover, compared to random collagen fibers with PDGFs, aligned collagen-NP fibers significantly promoted the desirable tenogenic differentiation of ADSCs, as evidenced by an increased level of tendon markers such as tenomodulin and scleraxis. On the other hand, no undesirable osteogenic differentiation, as measured by the unchanged level of alkaline phosphatase and osteocalcin, was observed. Together, these results indicate that the aligned collagen-NP composite fiber induced the tenogenic differentiation of ADSCs through both a topographic cue (aligned collagen fibril) and a chemical cue (PDGF released from NPs). Thus, our novel aligned collagen-NP composite fiber has a significant potential to be used for tendon tissue engineering and regeneration.

KEYWORDS:

Collagen; Growth Factors; Nanoparticle; Stem cell; Tendon

PMID:
24291329
DOI:
10.1016/j.actbio.2013.11.017
[Indexed for MEDLINE]
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