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Biosens Bioelectron. 2014 Apr 15;54:306-10. doi: 10.1016/j.bios.2013.10.043. Epub 2013 Nov 12.

IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies.

Author information

1
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
2
Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan. Electronic address: tlyang@ntu.edu.tw.
3
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan; Department of Electrical Engineering, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan. Electronic address: jjhuang@cc.ee.ntu.edu.tw.

Abstract

We demonstrate a biosensor structure consisting of an IGZO (Indium-Gallium-Zinc-Oxide) TFT (thin film transistor) and an extended sensing pad. The TFT acts as the sensing and readout device, while the sensing pad ensures the isolation of biological solution from the transistor channel layer, and meanwhile increases the sensing area. The biosensor is functionalized by first applying ZnO nanorods to increase the surface area for attracting electrical charges of EGFR (epidermal growth factor receptor) antibodies. The device is able to selectively detect 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml extracted from squamous cell carcinoma (SCC). Furthermore, the conjugation duration of the functionalized device with EGFR can be limited to 3 min, implying that the biosensor has the advantage for real-time detection.

KEYWORDS:

Biosensors; Nanorods; Thin film transistors

PMID:
24291267
DOI:
10.1016/j.bios.2013.10.043
[Indexed for MEDLINE]

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