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J Biomater Appl. 2014 Jul;29(1):46-58. doi: 10.1177/0885328213513934. Epub 2013 Nov 27.

Precipitation of hydroxyapatite on electrospun polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering.

Author information

1
Department of Textile Technology, Anna University, Chennai, India Centre for Biotechnology, Anna University, Chennai, India.
2
Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
3
Centre for Biotechnology, Anna University, Chennai, India.
4
Department of Textile Technology, Anna University, Chennai, India vrgiridev@yahoo.com.

Abstract

Advances in electrospun nanofibres with bioactive materials have enhanced the scope of fabricating biomimetic scaffolds for tissue engineering. The present research focuses on fabrication of polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds by electrospinning followed by hydroxyapatite deposition by calcium-phosphate dipping method for bone tissue engineering. Morphology, composition, hydrophilicity and mechanical properties of polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds along with controls polycaprolactone and polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds were examined by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and tensile tests, respectively. Adipose-derived stem cells cultured on polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds displayed highest cell proliferation, increased osteogenic markers expression (alkaline phosphatase and osteocalcin), osteogenic differentiation and increased mineralization in comparison with polycaprolactone control. The obtained results indicate that polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds have appropriate physico-chemical and biological properties to be used as biomimetic scaffolds for bone tissue regeneration.

KEYWORDS:

Aloe vera; bone tissue engineering; electrospinning; hydroxyapatite; silk fibroin

PMID:
24287981
DOI:
10.1177/0885328213513934
[Indexed for MEDLINE]

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