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BMB Rep. 2014 May;47(5):292-7.

Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.

Author information

1
Department of Ophthalmology, Konkuk University School of Medicine, Seoul 143-729, Korea.
2
Diatech Korea Co., Ltd, Seoul 138-826, Korea.
3
Department of Biomedical Science & Technology, Konkuk University, Seoul 143-729, Korea.

Abstract

Age-related macular degeneration (AMD) is the leading cause of blindness in the world. Evidence indicates that the suppression of the ubiquitin-proteasome system (UPS) contributes to the accumulation of toxic proteins and inflammation in retinal pigment epithelium (RPE), the functional abnormalities and/or the degeneration of which are believed to be the initiators and major pathologies of AMD. To identify new protein associations with the altered UPS in AMD, we used LC-ESI-MS/MS to perform a proteomic analysis of the aqueous humor (AH) of AMD patients and matched control subjects. Six UPS-related proteins were present in the AH of the patients and control subjects. Four of the proteins, including 26S proteasome non-ATPase regulatory subunit 1 (Rpn2), were increased in patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15 AMD patients compared to the control subjects, suggesting that this protein could be a biomarker for AMD.

PMID:
24286321
PMCID:
PMC4163863
DOI:
10.5483/bmbrep.2014.47.5.193
[Indexed for MEDLINE]
Free PMC Article

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