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Gene Ther. 2014 Feb;21(2):158-67. doi: 10.1038/gt.2013.68. Epub 2013 Nov 28.

Intratumoral gene therapy versus intravenous gene therapy for distant metastasis control with 2-diethylaminoethyl-dextran methyl methacrylate copolymer non-viral vector-p53.

Author information

1
Department of Pathology, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.
2
1] Department of Pulmonary, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece [2] Department of Interventional Pneumology, Ruhrlandklinik, West German Lung Center, University Hospital, University Duisburg-Essen, Essen, Germany.
3
Department of Pulmonary, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
4
Department of II Medical, "Coburg" Regional Department, University of Wuerzburg, Coburg, Germany.
5
Department of Respiratory Diseases, Changhai Hospital/First Affiliated Hospital of the Second Military Medical University, Shanghai, China.
6
Interventional Drug Delivery Systems and Strategies (ID2S2), Medical Cryogenics, Lakeland Court Jupiter, Jupiter, FL, USA.
7
Department of Biochemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
8
Department of Histology-Embryology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
9
Department of Cardiothoracic Surgery, "Saint Luke" Private Hospital of Health Excellence, Thessaloniki, Greece.
10
Experimental Animal Laboratory, "G. Papanikolaou" General Hospital, Thessaloniki, Greece.

Abstract

Lung cancer still remains to be challenged by novel treatment modalities. Novel locally targeted routes of administration are a methodology to enhance treatment and reduce side effects. Intratumoral gene therapy is a method for local treatment and could be used either in early-stage lung cancer before surgery or at advanced stages as palliative care. Novel non-viral vectors are also in demand for efficient gene transfection to target local cancer tissue and at the same time protect the normal tissue. In the current study, C57BL/6 mice were divided into three groups: (a) control, (b) intravenous and (c) intatumoral gene therapy. The novel 2-Diethylaminoethyl-Dextran Methyl Methacrylate Copolymer Non-Viral Vector (Ryujyu Science Corporation) was conjugated with plasmid pSicop53 from the company Addgene for the first time. The aim of the study was to evaluate the safety and efficacy of targeted gene therapy in a Lewis lung cancer model. Indeed, although the pharmacokinetics of the different administration modalities differs, the intratumoral administration presented increased survival and decreased distant metastasis. Intratumoral gene therapy could be considered as an efficient local therapy for lung cancer.

PMID:
24285215
DOI:
10.1038/gt.2013.68
[Indexed for MEDLINE]

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