Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):E4950-9. doi: 10.1073/pnas.1320301110. Epub 2013 Nov 26.

Nfatc1 orchestrates aging in hair follicle stem cells.

Author information

1
Laboratory of Mammalian Cell Biology and Development and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065.

Abstract

Hair production is fueled by stem cells (SCs), which transition between cyclical bouts of rest and activity. Here, we explore why hair growth wanes with age. We show that aged hair follicle SCs (HFSCs) in mice exhibit enhanced resting and abbreviated growth phases and are delayed in response to tissue-regenerating cues. Aged HFSCs are poor at initiating proliferation and show diminished self-renewing capacity upon extensive use. Only modestly restored by parabiosis, these features are rooted in elevated cell-intrinsic sensitivity and local elevation in bone morphogenic protein (BMP) signaling. Transcriptional profiling presents differences consistent with defects in aged HFSC activation. Notably, BMP-/calcium-regulated, nuclear factor of activated T-cell c1 (NFATc1) in HFSCs becomes recalcitrant to its normal down-regulating cues, and NFATc1 ChIP-sequencing analyses reveal a marked enrichment of NFATc1 target genes within the age-related signature. Moreover, aged HFSCs display more youthful levels of hair regeneration when BMP and/or NFATc1 are inhibited. These results provide unique insights into how skin SCs age.

KEYWORDS:

BMP signaling; hair cycle; quiescence

PMID:
24282298
PMCID:
PMC3870727
DOI:
10.1073/pnas.1320301110
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center