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J Appl Microbiol. 2014 Mar;116(3):542-53. doi: 10.1111/jam.12403. Epub 2013 Dec 11.

Degradation of dibenzofuran via multiple dioxygenation by a newly isolated Agrobacterium sp. PH-08.

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1
School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea.

Abstract

AIMS:

To demonstrate the biodegradation of dibenzofuran (DF) and its structural analogs by a newly isolated Agrobacterium sp. PH-08.

METHODS AND RESULTS:

To assess the biodegradation potential of newly isolated Agrobacterium sp. PH-08, various substrates were evaluated as sole carbon sources in growth and biotransformation experiments. ESI LC-MS/MS analysis revealed the presence of angular degrading by-products as well as lateral dioxygenation metabolites in the upper pathway. The metabolites in the lower pathway also were detected. In addition, the cometabolically degraded daughter compounds of DF-related compounds such as BP and dibenzothiophene (DBT) in dual substrate degradation were observed. Strain PH-08 exhibited the evidence of meta-cleavage pathway as confirmed by the activity and gene expression of catechol-2,3-dioxygenase.

CONCLUSIONS:

Newly isolated bacterial strain, Agrobacterium sp. PH-08, grew well with and degraded DF via both angular and lateral dioxygenation as demonstrated by metabolites identified through ESI LC-MS/MS and GC-MS analyses. The other heterocyclic pollutants were also cometabolically degraded.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Few reports have described the complete degradation of DF by a cometabolic lateral pathway. Our study demonstrates the novel results that the newly isolated strain utilized the DF as a sole carbon source and mineralized it via multiple dioxygenation.

KEYWORDS:

Agrobacterium sp. PH-08; angular and lateral dioxygenation; cometabolism; dibenzofuran

PMID:
24279899
DOI:
10.1111/jam.12403
[Indexed for MEDLINE]
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