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PLoS One. 2013 Nov 22;8(11):e79813. doi: 10.1371/journal.pone.0079813. eCollection 2013.

The USP21 short variant (USP21SV) lacking NES, located mostly in the nucleus in vivo, activates transcription by deubiquitylating ubH2A in vitro.

Author information

1
Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, Japan ; Department of Surgery, Hiroshima University School of Medicine, Hiroshima, Japan.

Abstract

USP21 is a deubiquitylase that catalyzes isopeptide bond hydrolysis between ubiquitin and histone H2A. Since ubiqutylated H2A (ubH2A) represses transcription, USP21 plays a role in transcriptional activation. On the other hand, the localization of USP21 suggests it has an additional function in the cytoplasm. Here, we identified a USP21 short variant (USP21SV) lacking a nuclear export signal (NES). Differential localization of USP21SV, more in the nucleus than the USP21 long variant (USP21LV), suggests they have redundant roles in the cell. Ectopic expression of both USP21 variants decreased ubH2A in the nucleus. Furthermore, both recombinant USP21 variants activate transcription by deubiquitylating ubH2A in vitro. These data suggest multiple roles for USP21 in the ubiquitylation-deubiquitylation network in the cell.

PMID:
24278184
PMCID:
PMC3838379
DOI:
10.1371/journal.pone.0079813
[Indexed for MEDLINE]
Free PMC Article

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