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Biosens Bioelectron. 2014 Apr 15;54:181-8. doi: 10.1016/j.bios.2013.10.048. Epub 2013 Nov 8.

Specific rare cell capture using micro-patterned silicon nanowire platform.

Author information

1
Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: sangkwonlee@cau.ac.kr.
2
Basic Research Laboratory (BRL), Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.
3
Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
4
Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA.
5
Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA; Yale Comprehensive Cancer Center, New Haven, CT 06520, USA.

Abstract

We report on the rapid and direct quantification of specific cell captures using a micro-patterned streptavidin (STR)-functionalized silicon nanowire (SiNW) platform, which was prepared by Ag-assisted wet chemical etching and a photo-lithography process. This platform operates by high-affinity cell capture rendered by the combination of antibody-epithelial cell surface-binding, biotin-streptavidin binding, and the topologically enhanced cell-substrate interaction on a 3-dimensional SiNWs array. In this work, we developed a micro-patterned nanowire platform, with which we were able to directly evaluate the performance enhancement due to nanotopography. An excellent capture efficiency of ~96.6±6.7%, which is the highest value achieved thus far for the targeting specific A549 cells on a selective area of patterned SiNWs, is demonstrated. Direct comparison between the nanowire region and the planar region on the same substrate indicates dramatically elevated cell-capture efficiency on nanotopological surface identical surface chemistry (<2% cell-capture efficiency). An excellent linear response was seen for quantifying captured A549 cells with respect to loaded cells. This study suggests that the micro-patterned STR-functionalized SiNWs platform provides additional advantage for detecting rare cells populations in a more quantitative and specific manner.

KEYWORDS:

Biocompatibility; Cell capture; Circulating tumor cells (CTCs); Filopodia; Silicon nanowires; Streptavidin functionalization

PMID:
24274988
DOI:
10.1016/j.bios.2013.10.048
[Indexed for MEDLINE]

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