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ACS Chem Biol. 2014 Feb 21;9(2):334-8. doi: 10.1021/cb400704n. Epub 2013 Nov 25.

Prometastatic GPCR CD97 is a direct target of tumor suppressor microRNA-126.

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1
Division of Chemistry and Chemical Engineering, MC 210-41, California Institute of Technology , 1200 East California Boulevard, Pasadena, California 91125, United States.

Abstract

Tumor suppressor microRNA-126 (miR-126) is often down-regulated in cancer cells, and its overexpression is found to inhibit cancer metastasis. To elucidate the mechanism of tumor suppression by miR-126, we analyzed the proteomic response to miR-126 overexpression in the human metastatic breast cancer cell line MDA-MB-231. To acquire quantitative, time-resolved information, we combined two complementary proteomic methods, BONCAT and SILAC. We discovered a new direct target of miR-126: CD97, a pro-metastatic G-protein-coupled receptor (GPCR) that has been reported to promote tumor cell invasion, endothelial cell migration, and tumor angiogenesis. This discovery establishes a link between down-regulation of miR-126 and overexpression of CD97 in cancer and provides new mechanistic insight into the role of miR-126 in inhibiting both cell-autonomous and non-cell-autonomous cancer progression.

PMID:
24274104
PMCID:
PMC3944050
DOI:
10.1021/cb400704n
[Indexed for MEDLINE]
Free PMC Article
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