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Nat Methods. 2014 Jan;11(1):47-50. doi: 10.1038/nmeth.2734. Epub 2013 Nov 24.

A quantitative liposome microarray to systematically characterize protein-lipid interactions.

Author information

1
1] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [2].
2
Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
3
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
4
Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
5
1] Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada. [2].
6
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

PMID:
24270602
DOI:
10.1038/nmeth.2734
[Indexed for MEDLINE]

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