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Toxicol In Vitro. 2014 Mar;28(2):182-6. doi: 10.1016/j.tiv.2013.11.003. Epub 2013 Nov 20.

Lichen secondary metabolites as DNA-interacting agents.

Author information

1
Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia.
2
Institute of Biophysics, Department of Molecular Biophysics and Pharmacology, Academy of Science of the Czech Republic, Brno, Czech Republic.
3
Institute of Biology and Ecology, Department of Botany, Pavol Jozef Šafárik University in Košice, Slovakia.
4
Institute of Chemistry, Department of Biochemistry, Pavol Jozef Šafárik University in Košice, Slovakia. Electronic address: maria.kozurkova@upjs.sk.

Abstract

A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).

KEYWORDS:

DNA-binding; Lichens; Topoisomerase I, II

PMID:
24269500
DOI:
10.1016/j.tiv.2013.11.003
[Indexed for MEDLINE]

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