Format

Send to

Choose Destination
See comment in PubMed Commons below
Dev Cell. 2013 Dec 9;27(5):586-97. doi: 10.1016/j.devcel.2013.09.029. Epub 2013 Nov 21.

Spindle formation in the mouse embryo requires Plk4 in the absence of centrioles.

Author information

1
Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK; Pluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
2
Department of Evolutionary Biology, University of Siena, Via A. Moro 41-53100, Siena, Italy.
3
Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
4
Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. Electronic address: dmg25@mole.bio.cam.ac.uk.
5
Pluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK. Electronic address: mz205@cam.ac.uk.

Abstract

During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, is also essential for spindle assembly in the absence of centrioles. Depletion of maternal Plk4 prevents nucleation and growth of microtubules and results in monopolar spindle formation. This leads to cytokinesis failure and, consequently, developmental arrest. We show that Plk4 function depends on its kinase activity and its partner protein, Cep152. Moreover, tethering Cep152 to cellular membranes sequesters Plk4 and is sufficient to trigger spindle assembly from ectopic membranous sites. Thus, the Plk4-Cep152 complex has an unexpected role in promoting microtubule nucleation in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.

PMID:
24268700
PMCID:
PMC3898710
DOI:
10.1016/j.devcel.2013.09.029
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center