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Anal Chim Acta. 2013 Dec 4;804:313-20. doi: 10.1016/j.aca.2013.10.037. Epub 2013 Oct 25.

Ionic liquids monolithic columns for protein separation in capillary electrochromatography.

Author information

1
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.

Abstract

A series of ionic liquids (ILs) monolithic capillary columns based on 1-vinyl-3-octylimidazolium (ViOcIm(+)) were prepared by two approaches ("one-pot" approach and "anion-exchange" approach). The effects of different anions (bromide, Br(-); tetrafluoroborate, BF4(-); hexafluorophosphate, PF6(-); and bis-trifluoromethanesulfonylimide, NTf2(-)) on chromatography performance of all the resulting columns were investigated systematically under capillary electrochromatography (CEC) mode. The results indicated that all these columns could generate a stable reversed electroosmotic flow (EOF) over a wide pH range from 2.0 to 12.0. For the columns prepared by "one-pot" approach, the EOF decreased in the order of ViOcIm(+)Br(-)>ViOcIm(+)BF4(-)>ViOcIm(+)PF6(-)>ViOcIm(+)NTf2(-) under the same CEC conditions; the ViOcIm(+)Br(-) based column exhibited highest column efficiencies for the test small molecules; the ViOcIm(+)NTf2(-) based column possessed the strongest retention for aromatic hydrocarbons; and baseline separation of four standard proteins was achieved on ViOcIm(+)NTf2(-) based column corresponding to the highest column efficiency of 479,000 N m(-1) for cytochrome c (Cyt c). These results indicated that the property of ILs based columns could be tuned successfully by changing anions, which gave these columns potential to separate both small molecules and macro biomolecules.

KEYWORDS:

1-vinyl-3-octylimidazolium bis-trifluoromethanesulfonylimide; 1-vinyl-3-octylimidazolium bromide; 1-vinyl-3-octylimidazolium hexafluorophosphate; 1-vinyl-3-octylimidazolium tetrafluoroborate; Anion effect; CE; CEC; Capillary electrochromatography; EOF; GC; HPLC; ILs; Ionic liquid; Monolithic column; PIL; Protein separation; RTILs; SEM; ViOcIm(+)BF(4)(−); ViOcIm(+)Br(−); ViOcIm(+)NTf(2)(−); ViOcIm(+)PF(6)(−); capillary electrochromatography; capillary electrophoresis; electroosmotic flow; gas chromatography; high performance liquid chromatography; ionic liquids; polymeric ionic liquid; room temperature ionic liquids; scanning electron microscopy

PMID:
24267098
DOI:
10.1016/j.aca.2013.10.037
[Indexed for MEDLINE]
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