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Food Chem. 2014 Apr 1;148:184-7. doi: 10.1016/j.foodchem.2013.09.137. Epub 2013 Oct 11.

Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935.

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1
Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, Seoul 120-749, Republic of Korea.

Abstract

A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichiacoli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 °C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.

KEYWORDS:

Anti-fungal; Candida albicans; Curdlan; Endo-β-1,3-glucanase; β-1,3-Glucooligosaccharide

PMID:
24262544
DOI:
10.1016/j.foodchem.2013.09.137
[Indexed for MEDLINE]
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