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Minimum Significant Ratio – A Statistic to Assess Assay Variability.

Source

Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.
2013 Nov 1 [updated 2017 Nov 20].

Author information

1
National Center for Advancing Translational Sciences, National Institutes of Health
2
University of California, San Francisco
3
Novartis Institutes for Biomedical Research
4
Monash Institute of Pharmaceutical Sciences
5
Western Michigan University
6
Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute
7
Brigham and Women's Hospital
8
Charles River Laboratories
9
Pfizer
10
Orig3n
11
Eli Lilly & Company
12
Bristol-Myers Squibb
13
Q-State Biosciences
14
Promega Corporation
15
PerkinElmer, Inc.
16
QualSci Consulting, LLC
17
Eli Lilly and Company
18
AbbVie

Excerpt

The MSR is a statistical parameter that characterizes the reproducibility of potency estimates from in vitro concentration-response assays. Biological activity expressed as potency of compounds is an important parameter in screening and drug discovery. Standard potency estimates are expressed as IC50, EC50 or simply as AC50 values derived from concentration-response (CRC) assays designed to measure activation, inhibition or modulation of targets and pathways of pharmacological significance. Concepts of Minimum Significant Ratio (MSR) discussed below address how the reproducibility of potency values is derived quantitatively using standard or control compounds of known activity and test compounds that have been tested in multiple runs. MSR values inform the scientists the extent of reliability of assays designed to support new compound selection through Structure-Activity Relationships (SAR) of chemical libraries.

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