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PLoS One. 2013 Nov 18;8(11):e81253. doi: 10.1371/journal.pone.0081253. eCollection 2013.

A genome-wide screen identifies yeast genes required for tolerance to technical toxaphene, an organochlorinated pesticide mixture.

Author information

1
Department of Nutritional Science and Toxicology, University of California, Berkeley, Berkeley, California, United States of America.

Abstract

Exposure to toxaphene, an environmentally persistent mixture of chlorinated terpenes previously utilized as an insecticide, has been associated with various cancers and diseases such as amyotrophic lateral sclerosis. Nevertheless, the cellular and molecular mechanisms responsible for these toxic effects have not been established. In this study, we used a functional approach in the model eukaryote Saccharomyces cerevisiae to demonstrate that toxaphene affects yeast mutants defective in (1) processes associated with transcription elongation and (2) nutrient utilization. Synergistic growth defects are observed upon exposure to both toxaphene and the known transcription elongation inhibitor mycophenolic acid (MPA). However, unlike MPA, toxaphene does not deplete nucleotides and additionally has no detectable effect on transcription elongation. Many of the yeast genes identified in this study have human homologs, warranting further investigations into the potentially conserved mechanisms of toxaphene toxicity.

PMID:
24260565
PMCID:
PMC3832591
DOI:
10.1371/journal.pone.0081253
[Indexed for MEDLINE]
Free PMC Article

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