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J Surg Res. 2014 Mar;187(1):297-301. doi: 10.1016/j.jss.2013.10.024. Epub 2013 Oct 18.

New method of stent-facilitated arterial reconstruction for orthotopic mouse liver transplantation.

Author information

  • 1Division of Transplantation Surgery, Department of Surgery, Medical University of South Carolina, Charleston, South Carolina.
  • 2Department of Surgery, Yale School of Medicine, New Haven, Connecticut.
  • 3Division of Transplantation Surgery, Department of Surgery, Medical University of South Carolina, Charleston, South Carolina. Electronic address: chavinkd@musc.edu.

Abstract

BACKGROUND:

Arterialized orthotopic liver transplantation (OLT) in the mouse mimics human liver transplantation physiologically and clinically. The present method of sutured anastomosis for reconstruction of the hepatic artery is complex and is associated with high incidence of complications and failure. This makes the endpoint assessment of using this complex model difficult because of the many variables of the technical aspect.

METHODS:

A total of 14 pairs of donors and recipients from syngeneic male mice were used for arterialized OLT. The grafts were stored in University of Wisconsin solution at 4°C for less than 4 h, and the recipients underwent OLT using a two-cuff technique. The arterial reconstruction was facilitated by the use of a single stent connecting the donor liver artery segment to the recipient common hepatic artery.

RESULTS:

All 14 recipients survived with the time for arterial reconstruction ranging from 4-10 min. Patency of the artery was confirmed by transecting the artery near the graft 2 and 14 d after transplantation. At day 2, five of the six arteries transected were patent and at day 14, seven of the remaining eight were patent for an overall patency rate of 85.7%.

CONCLUSIONS:

The stent-facilitated arterial reconstruction can be done quickly with a high patency rate. This model expands the translational research efforts to address marginal livers such as steatotic livers.

KEYWORDS:

Anastomosis; Arterialized; Liver; Mouse model; OLT; Obesity; Stent; Transplant

PMID:
24252852
PMCID:
PMC3960586
DOI:
10.1016/j.jss.2013.10.024
[PubMed - indexed for MEDLINE]
Free PMC Article
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