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Chem Phys Lipids. 2014 Jan;177:41-50. doi: 10.1016/j.chemphyslip.2013.11.001. Epub 2013 Nov 16.

Metabolic incorporation of unsaturated fatty acids into boar spermatozoa lipids and de novo formation of diacylglycerols.

Author information

1
Institute for Reproduction of Farm Animals Schönow e.V., Bernauer Allee 10, D-16321 Bernau, Germany; Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, D-10315 Berlin, Germany.
2
Humboldt-University Berlin, Department of Biology, Invalidenstr. 42, D-10115 Berlin, Germany.
3
University of Copenhagen, Department of Plant and Environmental Sciences, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
4
Institute for Reproduction of Farm Animals Schönow e.V., Bernauer Allee 10, D-16321 Bernau, Germany.
5
University of Leipzig, Department of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany. Electronic address: juergen.schiller@medizin.uni-leipzig.de.
6
Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, D-10315 Berlin, Germany. Electronic address: mueller@izw-berlin.de.

Abstract

Lipids play an important role in the maturation, viability and function of sperm cells. In this study, we examined the neutral and polar lipid composition of boar spermatozoa by thin-layer chromatography/mass spectrometry. Main representatives of the neutral lipid classes were diacylglycerols containing saturated (myristoyl, palmitoyl and stearoyl) fatty acyl residues. Glycerophosphatidylcholine and glycerophosphatidylethanolamine with alk(en)yl ether residues in the sn-1 position and unsaturated long chained fatty acyl residues in sn-2 position were identified as the most prominent polar lipids. The only glycoglycerolipid was sulfogalactosylglycerolipid carrying 16:0-alkyl- and 16:0-acyl chains. Using stable isotope-labelling, the metabolic incorporation of exogenously supplied fatty acids was analysed. Boar spermatozoa incorporated hexadecenoic (16:1), octadecenoic (18:1), octadecadienoic (18:2) and octadecatrienoic (18:3) acids primarily in the diacylglycerols and glycerophosphatidylcholines. In contrast, incorporation of eicosapentaenoic acid (20:5) was not detected. The analysis of molecular species composition subsequent to the incorporation of exogenous [(14)C]-octadecadienoic acid suggests two pathways for incorporation of exogenous fatty acids into glycerophosphatidylcholine: (1) de novo synthesis of glycerophosphatidylcholine via the CDP-choline pathway and (2) reacylation of lysophosphatidylcholine via an acyltransferase.

KEYWORDS:

AA; BTS; Beltsville Thawing Solution; Boar spermatozoa; DAG; DHB; Diacylglycerol; FFA; Fatty acid; GC; GPA; GPC; GPE; GPI; GPL; GPS; Glycerophospholipid; LPC; MAG; MS; PLA(2); PLC; SGG; TLC; aminoacridine; diacylglycerol; dihydroxybenzoic acid; free fatty acids; gas chromatography; glycerophosphatidic acid; glycerophosphatidylcholine; glycerophosphatidylethanolamine; glycerophosphatidylinositol; glycerophosphatidylserine; glycerophospholipid; lyso-glycerophosphatidylcholine (monoacylglycerophosphatidylcholine); mass spectrometry; monoacylglycerol; phospholipase A(2); phospholipase C; sulfogalactosylglycerolipid; thin-layer chromatography

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