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J Ethnopharmacol. 2014;151(1):601-8. doi: 10.1016/j.jep.2013.11.014. Epub 2013 Nov 16.

Effect of sulfated polysaccharides from Laminaria japonica on vascular endothelial cells in psychological stress rats.

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Department of Physiology, Guangxi Medical University, Nanning 530021, China.
Medical Scientific Research Center, Guangxi Medical University, Nanning 530021, China.
Department of Physiology, Guangxi Medical University, Nanning 530021, China. Electronic address:



Laminaria japonica is a popular seafood and medicinal plant in China. Laminaria japonica is used in traditional Chinese medicine to treat and prevent hypertension and edema.


The vascular protective activity and mechanism of sulfated polysaccharides were studied in adrenalin-induced vascular endothelial damage in rats after psychological stress (PS). Vehicle (sham and PS groups), sulfated polysaccharide from Laminaria japonica (LP; 1mg/kg and 5mg/kg) and enoxaparin sodium (1IU/kg, reference drug) were all administered for 10 days. Behavioral changes were recorded. Plasma levels of adrenalin, cortisol, monoamine oxidase (MAO), semicarbazide-sensitive amine oxidase (SSAO), formaldehyde, H2O2, nitric oxide (NO), endothelin-1 (ET-1), 6-keto-prostaglandin F1a (6-keto-PGF1a), and thromboxane B2 (TXB2) were measured. Endothelium-dependent relaxation of the thoracic aorta was measured and transmission electron microscopy of aortic vessels was performed.


Adrenalin metabolites in plasma were significantly lower (P<0.01) in rats after LP administration compared with those in the PS groups. The normalized ratios of plasma NO/ET-1 and 6-keto-PGF1a/TXB2 were maintained and endothelium-dependent relaxation of the aorta was greatly enhanced after LP treatment (P<0.05). Morphological alterations were observed in vascular endothelial cells (VECs) in PS rats, with a higher number of lysosomes and vague mitochondrial cristae compared with those in the sham group. However, these histopathological changes were markedly alleviated after LP treatment.


This study shows a protective effect of LP on VECs in PS rats. LP can regulate plasma levels of NO, ET-1, and 6-keto-PGF1a, enhance endothelium-dependent relaxation, and alleviate histopathological changes of lysosomes and mitochondria in VECs. The potential mechanism of LP on VECs in PS rats is related to its function of reducing metabolites of adrenalin.


Acetylcholine (PubChem CID: 187); Adrenalin; Adrenalin (PubChem CID: 5816); Cortisol (PubChem CID: 5754); Endothelin-1 (PubChem CID: 16212950); Formaldehyde (PubChem CID: 712); Hydrogen peroxide (PubChem CID: 784); Nitric oxide (PubChem CID: 145068); Psychological stress; Sulfated polysaccharide; Vascular endothelial cells

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