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Int J Biol Sci. 2013 Nov 16;9(10):1070-8. doi: 10.7150/ijbs.6662. eCollection 2013.

Cells isolated from human periapical cysts express mesenchymal stem cell-like properties.

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1. Unit of Maxillofacial Surgery, Calabrodental, Crotone, Italy; ; 2. Tecnologica Research Institute, Biomedical Section, Crotone, Italy.


We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73(+), CD90(+), CD105(+), CD13(+), CD29(+), CD44(+), CD45(-), STRO-1(+), CD146(+)) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast- and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.


Mesenchymal stem cells (MSCs); adipogenic differentiation; cyst; mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs); odontogenic cyst.; osteogenic differentiation; periapical inflammatory tissues

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