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Plant Cell Rep. 1986 Apr;5(2):81-4. doi: 10.1007/BF00269239.

Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.

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Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA.


The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.


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