Format

Send to

Choose Destination
J Biomed Opt. 2013 Nov;18(11):117010. doi: 10.1117/1.JBO.18.11.117010.

Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues.

Author information

1
UMR8165 Université Paris Sud, CNRS Laboratoire Imagerie et Modélisation en Neurobiologie et Cancérologie Paris Sud, Orsay Campus Bâtiment 440, 91405 Orsay, France.

Abstract

Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450- to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μa and reduced scattering coefficient μ's were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

PMID:
24247811
DOI:
10.1117/1.JBO.18.11.117010
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Society of Photo-Optical Instrumentation Engineers
Loading ...
Support Center