Modulation of urokinase plasminogen activator gene expression during the transition from quiescent to proliferative state in normal mouse cells

EMBO J. 1986 May;5(5):855-61. doi: 10.1002/j.1460-2075.1986.tb04295.x.

Abstract

We have investigated the regulation of urokinase (u-PA) mRNA in quiescent mouse fibroblasts and keratinocytes stimulated to divide by the addition of serum or epidermal growth factor (EGF), respectively. Serum stimulation of quiescent fibroblasts (BALB/c 3T3 or Swiss 3T3) results in an early and transient increase of u-PA mRNA level, which precedes by several hours the onset of DNA synthesis. A similar response is elicited by EGF stimulation of quiescent keratinocytes. The increase of u-PA mRNA parallels that of c-myc mRNA, does not require protein synthesis and is at least in part due to increase in template activity of the u-PA gene. Induction of terminal differentiation of mouse keratinocytes results in a decrease of u-PA mRNA which parallels the decrease of thymidine incorporation. In conclusion, variation in the level of u-PA mRNA is seen during G0/G1 transition and correlates with the proliferative state of these normal mouse cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Division
  • Epidermal Cells
  • Epidermis / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Genes*
  • Keratins
  • Kinetics
  • Mice
  • Mice, Inbred BALB C
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics*
  • Transcription, Genetic*
  • Urokinase-Type Plasminogen Activator / genetics*

Substances

  • RNA, Messenger
  • Keratins
  • Urokinase-Type Plasminogen Activator