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Psychopharmacology (Berl). 2014 Apr;231(7):1417-25. doi: 10.1007/s00213-013-3331-2. Epub 2013 Nov 19.

Endocannabinoids underlie reconsolidation of hedonic memories in Wistar rats.

Author information

1
Laboratory of Psychopharmacology, Department of Pharmacology, Universidade Federal de Santa Catarina, Campus Universitário Trindade, 88049-900, Florianopolis, SC, Brazil, decarvalhocr@gmail.com.

Erratum in

  • Psychopharmacology (Berl). 2014 Apr;231(7):1427.

Abstract

RATIONALE:

Drug addicts constantly relapse to drug seeking after recall of memories linked to the drug experience. It is believed that a successful application of therapies that block memory reconsolidation may end the continuous cycle of drug relapse.

OBJECTIVES:

The purpose of this study is to investigate whether modulation of the endocannabinoid system would impact the reconsolidation of opioid-related hedonic memories in rats previously paired to morphine context.

METHODS:

Male Wistar rats were trained to acquire a morphine-conditioned place preference (CPP). One week later, morphine-CPP memory was reactivated by a brief exposure to a drug-paired context. Immediately after the memory reactivation session, independent groups of morphine-trained rats received a single subcutaneous injection of different doses of cannabinoid CB1 receptor antagonist rimonabant, CB2-selective antagonist AM630, potent CB1/CB2 agonist WIN 55,212-2, inhibitor of enzyme fatty acid amide hydrolase URB597, or vehicle. Morphine-CPP was retested 1 and 2 weeks after reactivation.

RESULTS:

Blockade of CB1 (but not CB2) cannabinoid receptors impaired CPP reconsolidation of morphine-CPP at both tests 1 and 2 weeks post-reactivation, whereas direct activation of cannabinoid receptors did not produce significant effects on morphine-induced CPP. However, boosting endocannabinoid signaling by inhibition of anandamide metabolism promoted a transient CB1-dependent enhancement of the CPP.

PMID:
24247477
DOI:
10.1007/s00213-013-3331-2
[Indexed for MEDLINE]

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