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Bioresour Technol. 2014 Jan;151:249-57. doi: 10.1016/j.biortech.2013.10.070. Epub 2013 Oct 30.

Molecular characterization of anaerobic digester microbial communities identifies microorganisms that correlate to reactor performance.

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Agriculture and Agri-Food Canada, 107 Science Pl., Saskatoon, SK S7N 0X2, Canada; Department of Veterinary Microbiology, WCVM, University of Saskatchewan, 52 Campus Dr., Saskatoon, SK S7N 5B4, Canada.


A time-course analysis was conducted of thermophilic anaerobic digestion of dairy manure and wheat distillery thin stillage. Sequencing of chaperonin targets provided a phylogenetic survey of both bacteria and archaea in the digestate, along with an appraisal of the diversity of the reactor microbiome. A total of 1129 bacterial operational taxonomic units (OTU) were detected in the reactors, with OTU related to Clostridium becoming numerically dominant by day 7, and Acetivibrio-related OTU by day 35. Archaeal communities were less diverse, with 19 OTU detected representing both acetoclastic and hydrogenotrophic methanogens. Regardless of input material, the same organisms came to dominate the reactors, reflecting strong selective pressures present in the digesters. Principal coordinate analysis of the microbial communities showed that the bacterial communities clustered based on factors other than input material. Bacterial and archaeal OTU were identified with significant correlations to performance parameters, suggesting important roles in the methane production pathway.


Anaerobic digestion; Methanogenesis; Pyrosequencing; Syntrophic acetate oxidation

[Indexed for MEDLINE]

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