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Brain Behav Immun. 2014 Jul;39:87-98. doi: 10.1016/j.bbi.2013.10.028. Epub 2013 Nov 15.

Six weeks of voluntary wheel running modulates inflammatory protein (MCP-1, IL-6, and IL-10) and DAMP (Hsp72) responses to acute stress in white adipose tissue of lean rats.

Author information

1
Department of Integrative Physiology, University of Colorado at Boulder, USA.
2
Department of Integrative Physiology, University of Colorado at Boulder, USA; Center for Neuroscience, University of Colorado at Boulder, USA.
3
Department of Integrative Physiology, University of Colorado at Boulder, USA; Center for Neuroscience, University of Colorado at Boulder, USA. Electronic address: monika.fleshner@colorado.edu.

Abstract

To prime local tissues for dealing with potential infection or injury, exposure to an acute, intense stressor evokes increases in circulating and local tissue inflammatory proteins. Regular physical activity facilitates stress-evoked innate reactivity and modulates the expression of inflammatory proteins in immuno-metabolic tissues such as white adipose tissue (WAT). The impact of regular physical activity on stress-evoked inflammatory protein expression in WAT, however, remains unclear. To investigate this question, lean male F344 rats (150-175g) were allowed voluntary access to a running wheel for 6weeks followed by exposure to an acute stressor (100, 1.5mA-5s inescapable tail shocks). Using ELISAs, corticosterone, heat shock protein 72 (Hsp72), macrophage chemoattractant protein (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-6, and IL-10 concentrations were measured in plasma and subcutaneous, intraperitoneal (epididymal and retroperitoneal WAT depots) and visceral (omental and mesenteric WAT depots) WAT compartments. Acute stress increased plasma concentrations of all proteins except TNF-α and, depending upon the compartment examined, WAT concentrations of MCP-1, IL-1β, IL-6, and IL-10. Exercise ubiquitously increased IL-1β within WAT, potentiated stress-evoked Hsp72 in plasma and WAT, and differentially increased stress-evoked MCP-1, IL-6, and IL-10 within WAT. These data suggest: (a) inflammatory proteins in non-obese WAT may serve compartment-specific immune and metabolic roles important to the acute stress response and; (b) voluntary habitual exercise may optimize stress-induced augmentation of innate immune function through increases in stress-evoked Hsp72, MCP-1, IL-6, and IL-10 and decreases in IL-1β/IL10 and TNF-α/IL10 ratios within white adipose tissue.

KEYWORDS:

Acute stress; Cytokine; Heat shock protein 72; Innate immunity; Interleukin-10; Interleukin-1beta; Interleukin-6; Macrophage chemoattractant protein-1; Sterile inflammation; Visceral adipose tissue

PMID:
24246250
PMCID:
PMC4301739
DOI:
10.1016/j.bbi.2013.10.028
[Indexed for MEDLINE]
Free PMC Article
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