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PLoS One. 2013 Nov 14;8(11):e81554. doi: 10.1371/journal.pone.0081554. eCollection 2013.

ERK phosphorylation regulates sleep and plasticity in Drosophila.

Author information

1
Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America ; Department of Anatomy, Washington University Medical School, Saint Louis, Missouri, United States of America.

Abstract

Given the relationship between sleep and plasticity, we examined the role of Extracellular signal-regulated kinase (ERK) in regulating baseline sleep, and modulating the response to waking experience. Both sleep deprivation and social enrichment increase ERK phosphorylation in wild-type flies. The effects of both sleep deprivation and social enrichment on structural plasticity in the LNvs can be recapitulated by expressing an active version of ERK (UAS-ERK(SEM)) pan-neuronally in the adult fly using GeneSwitch (Gsw) Gsw-elav-GAL4. Conversely, disrupting ERK reduces sleep and prevents both the behavioral and structural plasticity normally induced by social enrichment. Finally, using transgenic flies carrying a cAMP response Element (CRE)-luciferase reporter we show that activating ERK enhances CRE-Luc activity while disrupting ERK reduces it. These data suggest that ERK phosphorylation is an important mediator in transducing waking experience into sleep.

PMID:
24244744
PMCID:
PMC3828275
DOI:
10.1371/journal.pone.0081554
[Indexed for MEDLINE]
Free PMC Article

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