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PLoS One. 2013 Nov 14;8(11):e80117. doi: 10.1371/journal.pone.0080117. eCollection 2013.

Monitoring of dynamic microbiological processes using real-time flow cytometry.

Author information

1
Department of Environmental Microbiology, Eawag - Swiss Federal Institute for Aquatic Science and Technology, Dübendorf, Switzerland ; Department of Environmental Systems Science, ETH Zürich, Zürich, Switzerland.

Abstract

We describe a straightforward approach to continuously monitor a variety of highly dynamic microbiological processes in millisecond resolution with flow cytometry, using standard bench-top instrumentation. Four main experimental examples are provided, namely: (1) green fluorescent protein expression by antibiotic-stressed Escherichia coli, (2) fluorescent labeling of heat-induced membrane damage in an autochthonous freshwater bacterial community, (3) the initial growth response of late stationary E. coli cells inoculated into fresh growth media, and (4) oxidative disinfection of a mixed culture of auto-fluorescent microorganisms. These examples demonstrate the broad applicability of the method to diverse biological experiments, showing that it allows the collection of detailed, time-resolved information on complex processes.

PMID:
24244624
PMCID:
PMC3828236
DOI:
10.1371/journal.pone.0080117
[Indexed for MEDLINE]
Free PMC Article

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