Format

Send to

Choose Destination
PLoS Genet. 2013 Nov;9(11):e1003922. doi: 10.1371/journal.pgen.1003922. Epub 2013 Nov 14.

Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots.

Author information

1
Institut Jean-Pierre Bourgin UMR1318 INRA-AgroParisTech, Versailles, France.

Abstract

The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hundreds of CO and NCO molecules in two different hotspot regions in Arabidopsis thaliana. This analysis revealed that COs are concentrated in regions of a few kilobases where their rates reach up to 50 times the genome average. The hotspots themselves tend to cluster in regions less than 8 kilobases in size with overlapping CO distribution. Non-crossover (NCO) events also occurred in the two hotspots but at very different levels (local CO/NCO ratios of 1/1 and 30/1) and their track lengths were quite small (a few hundred base pairs). We also showed that the ZMM protein MSH4 plays a role in CO formation and somewhat unexpectedly we also found that it is involved in the generation of NCOs but with a different level of effect. Finally, factors acting in cis and in trans appear to shape the rate and distribution of COs at meiotic recombination hotspots.

PMID:
24244190
PMCID:
PMC3828143
DOI:
10.1371/journal.pgen.1003922
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center