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Planta. 1986 Apr;167(4):521-6. doi: 10.1007/BF00391228.

Purification and properties of ascorbate free-radical reductase from potato tubers.

Author information

1
Istituto di Botanica, Università di Bari, I-70126, Bari, Italy.

Abstract

Ascrobate free-radical reductase (EC 1.6.5.4) from potato tubers was purified to apparent homogencity by a method which included ammonium-sulfate precipitation, gel filtration and chromatography on diethylaminoethyl cellulose and hydroxylapatite. Gel filtration and gel electrophoresis showed that the purified enzyme was monomeric with a molecular weight of about 42 000. Enzyme activity was heat lable and severely inhibited by thiol reagents. The Km values for enzyme substrates were estimated.

PMID:
24240368
DOI:
10.1007/BF00391228

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