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Nat Methods. 2014 Jan;11(1):100-5. doi: 10.1038/nmeth.2731. Epub 2013 Nov 17.

A holidic medium for Drosophila melanogaster.

Author information

1
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
2
Medical Research Council (MRC) Centre for Developmental Neurobiology, King's College London, London, UK.
3
Behavior and Metabolism Laboratory, Champalimaud Neuroscience Program, Champalimaud Centre for the Unknown, Lisbon, Portugal.
4
1] Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. [2].
5
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.
6
1] Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. [2] Max Planck Institute for Biology of Ageing, Köln, Germany.
7
Max Planck Institute for Biology of Ageing, Köln, Germany.
8
1] Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA. [2] Geriatrics Center and Nathan Shock Center of Excellence in the Basic Biology of Aging, University of Michigan, Ann Arbor, Michigan, USA.

Erratum in

  • Nat Methods. 2014 Sep;11(9):971.
  • Nat Methods. 2015 Nov;12(11):1098.

Abstract

A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid-specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.

PMID:
24240321
PMCID:
PMC3877687
DOI:
10.1038/nmeth.2731
[Indexed for MEDLINE]
Free PMC Article

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