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Clin Res Hepatol Gastroenterol. 2014 Feb;38(1):63-72. doi: 10.1016/j.clinre.2013.08.014. Epub 2013 Nov 14.

Protective effects of L-carnitine, N-acetylcysteine and genistein in an experimental model of liver fibrosis.

Author information

1
Yuzuncu Yil University, Dursun Odabas Medical Center, Pediatric Gastroentrology, Van, Turkey. Electronic address: kaandemiroren@yahoo.com.
2
Yuzuncu Yil University, Dursun Odabas Medical Center, Pediatric Gastroentrology, Van, Turkey.

Abstract

AIM:

Liver fibrosis is a reversible wound-healing response that occurs following liver injury. In this study, we aimed to investigate the possible protective effects of L-carnitine, N-acetylcysteine and genistein in liver fibrosis induced by carbon tetrachloride (CCl4). In addition, the effects of these agents were compared in the same study.

METHODS:

In this study, rats were randomly allocated into 8 groups, consisting of 10 rats each, as follows: a control group, CCl4, L-carnitine, N-acetylcysteine, genistein, CCl4 and L-carnitine, CCl4 and N-acetylcysteine, and CCl4 and genistein. At the end of 6 weeks, blood and liver tissue specimens were collected. Alanine aminotransferase (ALT); aspartate aminotransferase (AST); complete blood count, tumor necrosis factor-α (TNF-α); platelet-derived growth factor-BB (PDGF-BB); interleukin-6 (IL-6); liver glutathione level; oxidant/antioxidant status; scores of hepatic steatosis, necrosis, inflammation, and fibrosis; and the expression of α-smooth muscle actin were studied.

RESULTS:

Although the ALT and AST values in the group administered CCl4 were significantly higher than in all the other groups (P<0.05), there was no significant difference between the control group and the groups administered CCl4 combined with L-carnitine, N-acetylcysteine and genistein (P>0.05). There were significant differences in the levels of TNF-α, PDGF-BB and IL-6 (P<0.05) between the CCl4 group and the groups with L-carnitine, N-acetylcysteine and genistein added to CCl4. N-acetylcysteine and genistein had positive effects on the oxidant/antioxidant status and on liver necrosis and fibrosis scores.

CONCLUSIONS:

In our study, L-carnitine, N-acetylcysteine and genistein showed significant protective effects in liver fibrosis induced by CCl4.

PMID:
24239319
DOI:
10.1016/j.clinre.2013.08.014
[Indexed for MEDLINE]

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