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Vet Microbiol. 2014 Jan 10;168(1):229-33. doi: 10.1016/j.vetmic.2013.10.017. Epub 2013 Oct 26.

Clinically relevant multidrug resistant Salmonella enterica in swine and meat handlers at the abattoir.

Author information

1
ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; CECA-ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Rua D. Manuel II Apartado 55412, 4051-401 Porto, Portugal. Electronic address: egomesneves@mail.icav.up.pt.
2
Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Rua Dr. Roberto Frias, 4200 Porto, Portugal; REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira n° 228, 4050-313 Porto, Portugal. Electronic address: patriciaantunes@fcna.up.pt.
3
CECA-ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Rua D. Manuel II Apartado 55412, 4051-401 Porto, Portugal; INSA, Instituto Nacional de Saúde Dr. Ricardo Jorge, Laboratório Nacional de Referência da Resistência Antimicrobiana, Av. Padre Cruz, 1649-016 Lisboa, Portugal. Electronic address: vera.manageiro@insa.min-saude.pt.
4
ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal. Electronic address: fgartner@ipatimup.pt.
5
CECA-ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Rua D. Manuel II Apartado 55412, 4051-401 Porto, Portugal; INSA, Instituto Nacional de Saúde Dr. Ricardo Jorge, Laboratório Nacional de Referência da Resistência Antimicrobiana, Av. Padre Cruz, 1649-016 Lisboa, Portugal. Electronic address: manuela.canica@insa.min-saude.pt.
6
CECA-ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Rua D. Manuel II Apartado 55412, 4051-401 Porto, Portugal; INSA, Instituto Nacional de Saúde Dr. Ricardo Jorge, Laboratório Nacional de Referência da Resistência Antimicrobiana, Av. Padre Cruz, 1649-016 Lisboa, Portugal. Electronic address: jose.costa@insa.min-saude.pt.
7
REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira n° 228, 4050-313 Porto, Portugal. Electronic address: lpeixe@ff.up.pt.

Abstract

The presence of multidrug resistant (MDR) Salmonella serotypes in slaughtered swine, carcasses, meat and meat handlers is scarcely evaluated. Recently we demonstrated that diverse Salmonella serotypes are frequently present in swine, pork meat and carcasses, and meat handlers at Portuguese abattoirs. Here we have characterized their antibiotic resistance phenotypes and genotypes, helping elucidate the flow of MDR Salmonella in the food chain. Testing 60 Salmonella isolates from different serotypes, the highest frequencies of resistance were observed for tetracycline (T) [70% (n=42/60), tet(A)/tet(B)/tet(G)], streptomycin (S) [63% (n=38/60), aadA2/strA/strB], sulfamethoxazole (Sul) [62% (n=37/60), sul1/sul2/sul3] and ampicillin (A) [57% (n=34/60), blaPSE-1/blaTEM]. Thirty-seven percent (n=22/60) carried class 1 integrons and multidrug resistance was frequently observed (63% n=38/60), including those serotypes common to human infections [S. Typhimurium 78% n=25/32; S. 4,[5],12:i:- 67% n=2/3; S. Rissen 75% (n=3/4); S. London 67% n=2/3; S. Derby 55%; n=6/11)]. The emergent S. 4,[5],12:i:- isolates were mostly characterized by ASSuT phenotype [blaTEM/strA-strB/sul2/tet(B)], typical of the European clone, while for the first time the ST phenotype [strA-strB-tet(A)-tet(B)] was also observed. Moreover, we report a first finding of a MDR phenotype in S. London [ANSSuT; blaTEM-strA-strB-sul2-tet(A)]. Our findings suggest that the abattoir environment and the slaughter operations seem not only to harbor MDR serotypes that originated in the pig reservoir, but also propagate them through cross-contamination processes, involving meat handlers. The present study suggests a probable relationship between swine and human salmonellosis throughout the food chain, which is of interest for epidemiological, animal health and public health purposes.

KEYWORDS:

Antibiotic resistance; Meat; S. Typhimurium monophasic variant; Salmonella; Swine

PMID:
24239169
DOI:
10.1016/j.vetmic.2013.10.017
[Indexed for MEDLINE]

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